元件指南

VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。

CAR-T相关结构域

姓名	描述	Application Notes	References	序列
CD8-leader	Leader signal peptide of T-cell surface glycoprotein CD8 alpha chain	Directs transport and localization of the protein to the T-cell surface.	Cell. 64:891 (1991); WO2014055771A1; US20160185862A1; US10144770B2	View
CD8-hinge	Hinge region of T-cell surface glycoprotein CD8 alpha chain	Connects scFv and the transmembrane region; enhances tumor recognition efficiency; improves the expansion of CAR-T cells.	Cell. 64:891 (1991); J Immunother. 28:203 (2005); BMJ Open. 6: e013904 (2016); J Hematol Oncol. 10: 68 (2017); Oncoimmunology. 7: e1368604 (2018)	View
CD28-hinge	Hinge region of the T-cell surface glycoprotein CD28	Connects scFv with the transmembrane region providing stability and flexibilty for efficient CAR expression and function; enhances efficiency of tumor recognition; improves expansion of CAR-T cells.	J Immunother. 32:689 (2009); Mol Ther. 25:2452 (2017)	View
CD8-TM	Transmembrane region of T-cell surface glycoprotein CD8 alpha chain	Anchors the CAR to the plasma membrane, bridges the extracellular hinge and antigen recognition domains with the intracellular signaling region.	Cell. 64:891 (1991); BMJ Open. 6: e013904 (2016); Oncoimmunology. 7: e1368604 (2018); WO2014055771A1; US20100105136A1	View
CD28-TM	Transmembrane region of T-cell-surface glycoprotein CD28	Anchors the CAR to the plasma membrane; enhances receptor expression and stability.	Hum Gene Ther. 24:259 (2013); Cancer Res. 74:6383 (2014); WO2014055771A1; US10144770B2	View

激活结构域

姓名	描述	Application Notes	References	序列
CD3zeta	Intracellular domain of the T cell receptor-CD3ζ chain	Acts as a stimulatory molecule for activating T cell-mediated immune response.	Cell. 64:891 (1991); Nat Rev Immunol. 2:512 (2002); WO2014055771A1	View

共刺激结构域

姓名	描述	Application Notes	References	序列
CD28	CD28 intracellular costimulatory domain	Improves overall survival, proliferation and persistence of activated CAR-T cells.	Nat Biotechnol. 20:70 (2002); J Immunother. 32:689 (2009); WO2014055771A1	View
4-1BB	4-1BB intracellular costimulatory domain	Improves overall survival, proliferation and persistence of activated CAR-T cells; further enhances cytotoxicity and durability when used in combination with CD28.	Leukemia. 18:676 (2004); Mol Ther. 17:1453 (2009); EP2904106A2	View

scFv

姓名	描述	Application Notes	References	序列
CD19-scFv	Single-chain variable fragment derived from CD19 antibody	Recognizes human CD19-expressing cells in an antigen-specific manner.	J Immunother. 32:689 (2009); Mol Ther. 17:1453 (2009)	View

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