Popular Vectors for Gene (eNpHR3.0/mCherry)

基因信息

基因名称eNpHR3.0/mCherry
类型ORF
子类Optogenetic protein
描述Third generation of Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with mCherry. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of mCherry, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of mCherry to facilitate cell surface expression of NpHR
应用注释Light activation of NpHR leads to cell hyperpolarization and inhibition of firing action potentials; increased peak photocurrent compared to original NpHR; no aggregation; low cellular toxicity; maximum excitation is 580 nm; maximum excitation and emission of mCherry are 587 nm and 610 nm, respectively.
参考文献Unpublished, provided by Karl Deisseroth through the UNC vector core
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载体描述	载体类型	标记基因	价格
pAAV[Exp]-CMV>eNpHR3.0/mCherry:WPRE	哺乳动物基因表达AAV载体	-	￥1180	查看载体
pAAV[Exp]-CAG>eNpHR3.0/mCherry:WPRE	哺乳动物基因表达AAV载体	-	￥1180	查看载体
pRP[Exp]-CAG>eNpHR3.0/mCherry	哺乳动物基因表达载体	-	￥1180	查看载体

载体描述	载体类型	标记基因	价格
eNpHR3.0/mCherry expression regular plasmid 载体	Mammalian Gene Expression Vector 指南		￥1180 起
eNpHR3.0/mCherry expression lentivirus 载体	Mammalian Gene Expression Lentiviral Vector 指南		￥1180 起
eNpHR3.0/mCherry expression adenovirus 载体	Mammalian Gene Expression Adenoviral Vector 指南		￥1180 起
eNpHR3.0/mCherry expression AAV病毒载体载体	Mammalian Gene Expression AAV Vector 指南	--	￥1180 起
eNpHR3.0/mCherry expression PiggyBac转座载体载体	Mammalian Gene Expression PiggyBac Vector 指南		￥1180 起

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